Figure 4. Differential 5-LO enzyme expression and LTB4 production in C57BL/6 and 129/Sv mice.
The expression of alox5, aloxp5 and Ltbr1 mRNA in PMs from C57BL/6 and sv129 (A) and PMs infected with H. capsulatum (B) for 6 h as described in the Material and Methods. (B) LTB4 production by PMs from C57BL/6 and 129/Sv after in vitro infection with H. capsulatum (MOI = 1∶5) was measured by ELISA. The data are expressed as the mean ± SEM from one representative experiment of a total of two experiments (n = 3 to 5/each experiment). *sv129 compared with C57BL/6. p<0.05 was considered significant.