Figure 1. Cross reactivity of ATN-658 with uPAR from various species.

A & B. The specificity of ATN-658 was measured by direct binding assays using uPAR expressing mouse melanoma (B16), human prostate carcinoma (PC-3) and African green monkey (COS-1) cells and biotin-labeled ATN-658. B. FACS analysis was performed using HeLa cells (expressing human uPAR), D-17 lung carcinoma cells (expressing canine uPAR and COS-1 cells. Each cell line was incubated with normal rabbit serum (NRS), a rabbit polyclonal antibody raised against a fragment of human uPAR (rDIIDIII), mouse IgG (mIgG) or ATN-658 and the appropriate FITC labeled secondary antibody.