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. 2014 Jan 21;9(1):e85432. doi: 10.1371/journal.pone.0085432

Figure 3. Monoclonal IgM inhibits gene transfer of Huh7 hepatocellular carcinoma cells.

Figure 3

In vitro gene transfer of 2.5×1010 vp of HDA-luciferase to Huh7 cells (MOI 50.000) in the presence of 2% fetal bovine serum and 5 µg of monoclonal IgM (mIgM) directed to an irrelevant antigen (non crossreactive human AE2 transporter), monoclonal IgG which specifically recognized human interferon alpha-5 or serum polyclonal IgM from adenovirus naïve mice (pIgM) as indicated. Relative content of vector DNA was assessed by real time PCR in quadruplicate samples. pIgM, polyclonal immunoglobulin M; mIgM, monoclonal immunoglobulin M, mIgG, monoclonal immunoglobulin G; HDA-luc, helper-dependent adenoviral vectors encoding luciferase. The experiment was repeated three times.