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. 2014 Jan 21;9(1):e85401. doi: 10.1371/journal.pone.0085401

Figure 2. Effects of immunization with SmCB or FhCL without adjuvant on immune responses, assessed 6 days post infection.

Figure 2

(a) Titres of peptidase specific IgG were measured in the sera of immunized mice by ELISA. The data shown is representative of 3 independent experiments and displays the inverse of the end point serum dilution. (b) The isotype of peptidase specific antibodies in the sera of mice was determined by ELISA. The data shown is representative of 5 independent experiments. Each column represents the delta mean absorbance ± the standard error around the mean (SD). (c) Spleens were harvested from mice that were infected following immunization with SmCB1 or a combination of SmCB1 and FhCL1. The quantity of cytokine (IL-4, IL-5, IL-13 and IFN-γ) released into culture media by spleen cells stimulated with SmCB1 in vitro was measured by ELISA. Supernatants from cells incubated in media only served as negative controls. Each column represents the mean ± SD.