Table 1. Protective effects of immunization with C-1 peptidases SmCB1 and FhCL1 alone and in combination with SG3PDH/PRX-MAP on male and female worm burdens and parasitological parameters.
| Experiment | Vaccine formulation | % Reduction in Worm burden* | % Reduction in eggs in liver | % Reduction in eggs in small intestine | ||
| Total | Males | Females | ||||
| 1. Single immunization | SmCB1 | 60.05 (<0.0001) | 60.29 (<0.0001) | 59.57 (<0.0001) | 26.63 (<0.0041) | 26.01 (<0.018) |
| SmCB1&SG3PDH/PRX-MAP | 83.79 (<0.0001)a | 88.23 (<0.0001) | 82.44 (<0.0001) | 54.68 (<0.0001) | 37.58 (0.0006) | |
| 2. Two immunizations | SmCB1 | 66.19 (<0.0001) | 65.88 (<0.0001) | 66.55 (<0.0001) | 51.30 (<0.0007) | 24.71 (NS) |
| SmCB1&SG3PDH/PRX-MAP | 75.00 (<0.0001) | 74.1 (<0.0001) | 75.60 (<0.0001) | 58.44 (0.0002) | 41.24 (0.0096) | |
| 3. Two immunizations | FhCL1 | 60.40 (<0.0001) | 60.67 (<0.0001) | 59.89 (<0.0001) | 34.99 (<0.0007) | 24.05 (NS) |
| FhCL1&SG3PDH/PRX-MAP | 73.43 (<0.0001) | 73.78 (<0.0001) | 72.91 (<0.0001) | 60.10 (<0.0001) | 49.38 (<0.0001) | |
| 4. Single immunization | SmCB1&FhCL1&SG3PDH/PRX-MAP | 56.45 (<0.0001) | 52.84 (<0.0001) | 61.73 (<0.0001) | 58.84 (<0.0001) | 65.0 (<0.0001) |
| 5. Two immunizations | SmCB1&FhCL1&SG3PDH/PRX-MAP | 66.39 (<0.0001) | 66.66 (<0.0001) | 65.97 (<0.0001) | 65.97 (<0.0001) | 69.32 (<0.0001) |
Mice (10–12/group) were immunized with SmCB1 or FhCL1 combined with rSG3PDH/PRX-MAP and challenged 2 (panels 1–3) or 3 (panels 4 and 5) weeks after the last immunization.
*
Percent reduction was evaluated by the formula: % reduction = mean number in infected controls−mean number in infected, immunized mice/mean number in infected controls×100.
a
P values as calculated by Student 2-tailed t-test. NS = not significant.