Table 2. Recovery of lung-stage schistosomules from unimmunized and immunized mice 6 days after the challenge infection.
Vaccine formulation | No. of mice tested | No. of larvae/mouse ± SD | P value |
PBS (controls) | 12 | 16.1±3.9 | - |
SmCB1 | 10 | 15.1±4.7 | NS |
FhCL1 | 8 | 15.1±3.1 | NS |
SmCB1+FhCL1 | 8 | 13.2±3.6 | NS |
SmCB1+SG3PDH/PRX MAP | 6 | 7.5±3.4 | 0.0003* |
For each experiment, lung-stage larvae were recovered from 2–3 mice per group, and counted on an individual mouse basis. Mean number ± SD around the mean of larvae retrieved from 2–6 repeat groups is shown. NS = not significant compared to PBS controls, as assessed using the Student 2-tailed t-test.
Immunization of mice with SG3PDH or PRX-MAP alone or emulsified with Freund's, alum, or Allison' adjuvant failed to elicit changes in number of lung-stage larvae recovered from immunized mice compared to adjuvant controls (data not shown).