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. 2014 Jan 21;9(1):e85641. doi: 10.1371/journal.pone.0085641

Table 2. Details of the data sets used for estimation of nucleotide substitution rate and time to the most recent common ancestor for Cauliflower mosaic virus.

Parameter Open reading frame
I–V VI
Best-fit substitution model GTR+I+Γ4 GTR+I+Γ4
Best-fit molecular clock model Relaxed Uncorrelated Exponential Relaxed Uncorrelated Exponential
Best-fit population growth model Exponential growth Constant size
Sequence length (nt) 5106 1269
No. of sequences 66 97
Sampling date range 1960–2010 1960–2012
Chain length (in millions) 100 100
TMRCAa (years) 491 (86–1270) 431 (113–886)
Substitution rate (nt/site/year) 1.71×10−4 (1.45×10−5–3.87×10−4) 5.81×10−4 (2.47×10−4–9.47×10−4)
dN/dSb 0.069 0.201
No. of variable sites 1074 448
a

Time to the most recent common ancestor.

b

Nonsynomymous (dN) and synonymous (dS) substitution (dN/dS) ratios were calculated for seven ORFs using the Pamilo-Bianchi-Li (PBL) method in MEGA v5 [56].