(A) LATS2 and β-catenin interacted when overexpressed in 293T cells.
(B) The kinase domain of LATS2 was not required for the interaction between β-catenin and LATS.
(C and D) The amino acids 250–575 of LATS2 were required for interaction with β-catenin.
(E) β-catenin-133–694 containing arm repeats interacted with LATS2. 293T cells were co-transfected with HA-LATS2 and a variety of β-catenin deletion mutants.
(F) Endogenous β-catenin interacted with LATS2 upon LiCl or Wnt-3a stimulation.
(G) GST-β-catenin fusion protein pulled down HA-LATS2 protein in vitro. See also Fig. S2.