Table 1. Spore Viability of Four-Spore Tetrads.
Strain | Genotype | % of viable spores | # of spores |
WHY3285 | ho::hisG/″, leu2::hisG/″, HIS4::LEU2-(BamHI)/his4-X::LEU2-(BamHI)–URA3 | 99 | 240 |
WHY6082 | WHY3285, pch2Δ::KanMX4/″ | 84 | 216 |
WHY6448 | WHY3285, red1Δ::KanMX4/″ | 0 | 216 |
WHY10491 | WHY3285, pch2Δ::KanMX4/″, red1Δ::KanMX4/″ | <1 | 288 |
WHY10532 | WHY3285, XRS2-13myc::KanMX4/″, red1Δ::KanMX4/″ | 0 | 216 |
WHY10533 | WHY3285, xrs2ΔN-13myc::KanMX4/″, red1Δ::KanMX4/″ | 0 | 216 |
WHY10535 | WHY3285, red1Δ::KanMX4/″, dot1Δ::KanMX4/″ | <1 | 220 |
WHY9523 | WHY3285, red1Δ::KanMX4/″, rec8Δ::leu2/″ | 0 | 144 |
WHY9174 | WHY3285, pph3Δ::KanMX4/″ | 51 | 216 |
WHY10541 | WHY3285, pph3Δ::KanMX4/″, red1Δ::KanMX4/″ | <1 | 216 |
Spore viabilities were determined following sporulation in liquid medium at 30°C.