Figure 2. Treatment with T₃ increases ZAG production in HepG2 cells.

(A) ZAG media from HepG2 cells treated over the course of 3 days with vehicle (ethanol) or T₃ (1, 10 and 100 nM) was measured by ELISA. Data are expressed as mean ± SD of triplicates. (B) ZAG mRNA levels from HepG2 cells treated as in A. Human 18S was amplified as an internal control, and values are expressed as percentage relative to the untreated cells. Data are expressed as mean ± SD of triplicates. (C) Western blot of ZAG and PPIA from extracts of HepG2 cells treated as in A.