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. 2014 Jan 21;9(1):e85753. doi: 10.1371/journal.pone.0085753

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© 2014 Simó et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) ZAG media from human adipocytes treated over the course of 3 days with vehicle (ethanol) or T₃ (10 and 100 nM) was measured by ELISA. Data are expressed as mean ± SD of triplicates. (B) ZAG mRNA levels from human adipocytes treated as in A. Human 18S was amplified as an internal control, and values are expressed as percentage relative to the untreated cells. Data are expressed as mean ± SD of triplicates.