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. 2014 Jan 21;9(1):e85779. doi: 10.1371/journal.pone.0085779

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© 2014 Liu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Western blot analysis of the full-length recombinant WSSV VP11 (VP11-V5) expressed in Sf9 cells using either (A) anti-VP11 antibody or (B) anti-V5 antibody. Lane 1: cell lysate of pDHsp/V5-His transfected Sf9 cells. Lane 2: cell lysate of pDHsp/VP11-V5-His transfected Sf9 cells. (C) Intact WSSV virions were subjected to detergent and NaCl treatment as indicated. After fractionation, the pellet (P) and supernatant (S) fractions were separated on SDS-PAGE and detected by Western blotting to produce profiles that are characteristic of envelope, tegument and nucleocapsid proteins (6). Three representative WSSV structural proteins are shown for comparison. Lane V is the untreated purified virus.