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. 2014 Jan 21;9(1):e85779. doi: 10.1371/journal.pone.0085779

Figure 5. Yeast two-hybrid screening results for all of the confirmed WSSV VP11 interaction proteins.

Figure 5

(A) to (M) VP11’s interactions with VP24, VP26, VP28, VP37, VP38A, VP38B, VP51B, VP60B, VP75, VP95, VP160B, VP664-7 and itself, respectively. (a) Yeast growth on medium lacking both Leu and Trp (DDO) indicates the presence of each respective pair of plasmids. (b) and (c) Yeast growth on low stringency (DDO/X/A) and high stringency (QDO/X/A) medium, respectively. The blue signals in (b) and (c) are due to the presence of X-α-Gal. The positive signals represent protein-protein interactions. The numbers around the plates indicate the bait and prey plasmids of the transformed yeast: 1, pGBKT7-53/pGADT7-T; 2, pGBKT7-Lam/pGADT7-T; 3, pGBKT7-VP11/pGADT7-VPxx; 4, pGBKT7-VP11/pGADT7; 5, pGBKT7/pGADT7-VPxx; 6, pGBKT7/pGADT7, with VPxx respectively standing for each of the WSSV structural proteins listed above.