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. 2014 Jan 21;9(1):e85808. doi: 10.1371/journal.pone.0085808

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© 2014 Hou et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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MSCs were transfected with a siRNA against the ANGPTL4 transcript. The cells were also transfected witn a non-targeting siRNA(siRNA-NT) as control. The siRNA-mediated transfection efficiency was demonstrated by western blot (A and B) and ELISA(C). (Each column represents the mean ± SD of three independent experiments; *P<0.05 vs.siRNA-ANGPTL4). Then MSCs were treated with hypoxia/SD for 24 hr. Cells under normal culture were used as control. Then the apoptosis was measured by FACS(D and E).(Each column represents the mean ± SD of three independent experiments; *P<0.05 vs. control; ▴P<0.05 vs. hypoxia/SD+siRNA-ANGPTL4).