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. 2014 Jan 21;9(1):e85840. doi: 10.1371/journal.pone.0085840

Figure 5. Inhibition of Nodal by Nodal-specific shRNA in MHCC97H and HCCLM3 cells 24, 48, and 72 h after transfection, respectively.

Figure 5

(A) Relative Nodal mRNA expression in MHCC97H cell measured by qPCR. (B) The expression level of Nodal protein in MHCC97H cell using Western blotting. (C) Relative Nodal mRNA expression in HCCLM3 cell measured by qPCR. (D) The expression level of Nodal protein in HCCLM3 cell using Western blotting. 1, control; 2, 24 h after transfection; 3, 48 h after transfection; 4, 72 h after transfection. Data are Mean ± SD of three replicates. * P<0.05.