Table 1. Bacterial strains and plasmidsa .
| Strain or plasmid | Genotype and description | Source or reference |
| E. coli strains | ||
| NADC 5570 | stx2+ and streptomycin-resistant E. coli O157:H7 | [45], [46] |
| strain 86-24 | ||
| NADC 6431 | Δstx2 derivative of NADC 5570 | [32] |
| NADC 6491 | Δhha mutant strain of NADC 6431 | [32] |
| NADC 6488 | ΔqseC mutant strain of NADC 6431 | This study |
| NADC 6528 | ΔqseC mutant strain of NADC 6491 | This study |
| TOP 10 | F- mcrA Δ (mrr-hsdRMS-mcrBC) Φ80lacZΔM15 | Life Technologies |
| ΔlacX74 recA1 araD139 Δ (ara-leu)7697 galU | ||
| galK rpsL (StrR) endA1 nupG | ||
| Plasmids | ||
| pSMART-LC | Cloning vector | Lucigen |
| pCRXL and pCR2.1 | Cloning vectors | Life Technologies |
| pAM450 | Plasmid with a temperature-sensitive origin of | [32], [46] |
| replication | ||
| pSM400 | pCRXL containing a 3.0 kb US-DS fragment for | This study |
| deleting the qseC gene | ||
| pSM488 | qseC deletion plasmid constructed by cloning a | This study |
| 3.0 kb US-DS fragment of pSM400 at the XbaI | ||
| site of pAM450 | ||
| pSM639 | pSMART-LC containing 3.51 kb fragment for | This study |
| complementing the qseC deletion mutant | ||
| pSM197R | pCR2.1 carrying a 630 bp DNA fragment | [32], [56] |
| encoding hha | ||
a
Detailed descriptions of the construction of bacterial strains and plasmids listed in this table are provided under material and methods section.