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. 2014 Jan 21;9(1):e85832. doi: 10.1371/journal.pone.0085832

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© 2014 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Schema of the treatment. The mice were treated with saline (Saline) or CsA from 1 week before the HI to 4 weeks (CsA-4w) or 10 weeks (CsA-10w) after the HI. The levels of serum HBsAg (B) and HBV DNA (C) in the mice at the indicated time points were detected. The cut off values of HBsAg and HBV DNA are displayed as solid and dashed lines, respectively. Liver samples were collected from the mice (n≥3) at the indicated time points. The HBV replicative intermediates (D) and HBcAg (E) in the liver were analyzed by Southern blotting and IHC (magnification 200×), respectively.