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. 2014 Jan 21;9(1):e85832. doi: 10.1371/journal.pone.0085832

Figure 5. Adoptive immune transfer in mice with HBV persistence induced by CsA treatment.

Figure 5

(A) Schema of the treatment. The mice were treated with CsA from 1 week before the HI to 10 weeks after the HI. Two weeks later, the mice were adoptive transferred with the splenocytes from naïve or pHBc-immunized mice. The levels of serum HBsAg (B), HBsAb (C), and HBcAb (D) in the mice were detected at the indicated time points. The cut off values of the HBsAg, HBsAb, and HBcAb are shown as dashed lines. (E) Liver samples were collected from the mice (n≥3) at week 24 post HI (12 weeks after the adoptive transfer), and intrahepatic HBcAg was detected by IHC (magnification 200×). (F) Splenocytes were prepared from the mice (n≥3) at week 24 post HI (12 weeks after the adoptive transfer), and pulsed with rHBsAg and rHBcAg, IFN-γ-producing cells were analyzed by ELISPOT, as described above. * P<0.05.