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. 2014 Jan 21;9(1):e85883. doi: 10.1371/journal.pone.0085883

Figure 1. CD2-Lmo2 transgenic mice develop T-ALL with high penetrance, long latency and upregulation of Lyl1.

Figure 1

(A) schematic shows structure of CD2-Lmo2 transgene used in pronuclear injection. Lower panel shows a Southern blot of tail genomic DNA digested with EcoRI which cuts once within the transgene and probed with Lmo2 cDNA. Red asterisk shows transgene band in equal intensity to other bands which represent the endogenous Lmo2 exons. (B) photo of CD2-Lmo2 transgenic mouse sacrificed due to T-ALL onset. T, thymus; H, hepatomegaly; L, lymphadenopathy; S, splenomegaly. (C) Survival analysis of CD2-Lmo2 transgenic mice (n = 24). (D) PCR analysis of Jβ2 T-cell receptor showed rearrangement in most T-ALL genomic DNA; germline configuration is shown by the presence of only a 2 kb band in the lane. (E) Lmo2 and Lyl1 mRNA were quantified by qRT-PCR on whole RNA isolated from 24 CD2-Lmo2 T-ALLs and shown relative to levels in normal thymus. Values shown are fold increase over normal thymus.