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. 2014 Jan 21;9(1):e85935. doi: 10.1371/journal.pone.0085935

Figure 4. Role of MSLN in cellular migration and invasion.

Figure 4

A. No effects observed in the wound-healing assay, following siRNA transfections. Confluent monolayers of Mero-14 cells transfected with 40 nM of siCtrl, or siMSLN-1, respectively. Two different experiments were carried out, each performed in triplicate. B . Trans-well cell invasion assay on Mero-14 cells transfected with 40 nM of the siCtrl (top), or siMSLN-1 (bottom). Pictures were taken using a fluorescence microscope at 10X magnification and are reported as negative of the originals to enhance the contrast between the background and the DAPI-stained cells. The bar chart shows the average of invasive cells (error bars represent SEM of two independent experiments, each done in triplicate, *P  =  0.0044).