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. 2014 Jan 21;9(1):e85923. doi: 10.1371/journal.pone.0085923

Figure 5. T lymphocyte profiles in cecal lymph nodes and spleen after DSS-induced colitis.

Figure 5

T cells were isolated from cecal lymph nodes (CLN, upper panel) and spleen (lower panel) at the end of the experiment, and the frequencies of CD4+CD69+ and CD4+Foxp3+ T cells, as percentage of CD4+ T cells, were assessed by flow cytometry. T-cell frequencies are presented relative to the average frequency observed in the PBS control group (range from different experiments given in parentheses for each T cell type) which received the value of 100. Upper panel, CLN: CD4+CD69+ (100 = 2.7 to 3.54), CD4+FoxP3+ (100 = 3.41 to 5.41). Lower panel, spleen: CD4+CD69+ (100 = 4.5 to 4.51), CD4+FoxP3+ (100 = 1.59 to 4.02). PBS, control without bacteria or DSS; DSS, DSS only, without bacteria; 327+DSS, DSS plus Lb CNRZ327. For CD4+CD69+ T cells, bars represent the mean ± SEM of one experiment, with 2 or 3 mice in the PBS control groups and 5 mice in each of the other groups. For CD4+FoxP3+ T cells, bars represent the mean ± SEM calculated across two independent experiments, with 3 to 5 mice per group in each experiment (combined sample sizes: 8 to 10 mice per treatment (colon) and 8 mice per treatment (spleen)). ** = p<0.01; *** = p<0.001; • = p = 0.053.