Skip to main content
. 2014 Jan 21;9(1):e85906. doi: 10.1371/journal.pone.0085906

Figure 2. Effect of Sfrp5 on adipokine and myokine release from cytokine-stimulated cells.

Figure 2

Primary human adipocytes (A–C) and primary human skeletal muscle cells (D–E) were exposed to Sfrp5 (4 h; 100 ng/ml) prior to incubation with TNFα (24 h; 5 nmol/l) or MCP-1 (18 h; 2 ng/ml). Cytokine release by the adipocytes and myotubes was quantified by ELISA, and expressed as mean ± standard error of the mean (A–C: n = 5; D–E: n = 4). Differences among the various conditions were analyzed by Friedman's test followed by Dunn's multiple comparison test; ### indicates P<0.001; ##, P<0.01 versus cells kept untreated (basal); *, P<0.05 for the effect of the additional Sfrp5 incubation versus TNFα alone.