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. 2014 Jan 21;9(1):e85906. doi: 10.1371/journal.pone.0085906

Figure 5. Effect of Sfrp5 and TNFα on inflammation signaling in primary human adipocytes.

Figure 5

Primary human adipocytes were kept untreated, exposed to Sfrp5 for 24/ml TNFα for 24 h with or without a 4 h preincubation with Sfrp5. Cell lysates were analyzed for phosphorylation of NFκB-Ser536 (A) and JNK-Tyr183/Thr185 (B) by Western blotting. Phosphorylation signals were normalized for GAPDH protein abundance and expressed as mean ± standard error of the mean of seven independent experiments using cells from different donors. The values obtained for untreated cells were considered as control and set at 100%. Differences among groups were calculated by ANOVA followed by Bonferroni multiple comparison analysis. ***, p<0.001 versus untreated cells; ††, p<0.01 for the effect of Sfrp5 versus TNFα.