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. 2014 Jan 21;9(1):e86078. doi: 10.1371/journal.pone.0086078

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© 2014 Bayer et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Left panel (A, D, G, J): Effect of de-tensioning, mid panel (B, E, H, K): Effect of TGF-β1 on tensioned 3D tendon-constructs, right panel (C, F, I, L): Effect of TGF-β1 on de-tensioned 3D tendon-constructs. The data are presented on a logarithmic y scale as geometric means ± SD (n = 3) where Tension Day 1 is baseline. Significant 2-way RM ANOVA (tension*time) or (TGF-β1*time) main effects written above the graphs. Integrin α₂ (ITGA2); Integrin α₅ (ITGA5); Integrin α₁₁ (ITGA11); Integrin β₁ (ITGB1). Open triangles: 3D tendon-construct under tension, without TGF-β1 supplementation; open circles: 3D tendon-construct de-tensioned, without TGF-β1 supplementation; filled triangles: 3D tendon-construct under tension, with TGF-β1 supplementation; filled circles: 3D tendon-construct de-tensioned, with TGF-β1 supplementation.