Table 2. Summary of mRNA expression of tendon-related genes as a response to changes in the spatial/mechanical environment and TGF-β1 supplementation.

Target	Effect of 2D culture	Effect of de-tensioning	Effect of TGF-β1 supplementation
TNMD	↓	↓	↔
SCX	(↓)	↔	↑
MKX	↓	↓	↔
COL1A1	↓	↓	↔
COL3A1	↓	↓	↔
COL12A1	↔	↓	↔
COL14A1	↓	↓	↓
FN	↔	↔	↑
DCN	↓	↓	↓
FBMD	↓	Interaction tension x TGF-β1	Interaction tension x TGF-β1
TGFB1	↔	↑	↔
CCN2	(↑)	Interaction tension x TGF-β1	Interaction tension x TGF-β1
GAPDH	↑	↑	↑

Summary of figure 2 and 3: The effect of the intervention is compared to the gene expression of the respective target cultured for six days under static tension, the arrows (↑↓) indicate significant changes relative to the tensioned samples, or no statistical change (↔). Arrows in parenthesis indicate a tendency (p< 0.1). Tenomodulin (TNMD); Scleraxis (SCX); Mohawk homeobox (MKX); Collagen type I (COL1A1); collagen type III (COL3A1); collagen type XII (COL12A1); collagen type XIV (COL14A1); Fibronectin (FN); Decorin (DCN); Fibromodulin (FBMD); Transforming growth factor-β1 (TGFB1); Connective tissue growth factor (CCN2); Glyceraldehyde 3-phosphate dehydrogenase (GAPDH).