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. 2014 Jan 21;9(1):e86129. doi: 10.1371/journal.pone.0086129

Figure 7. Different TEC subsets are temporally regulated during postnatal thymus development in wild type mice.

Figure 7

(A) UEA/MHCII→K8/K5 (blue dots) and K8/K5→UEA/MHCII (red dots) overlays were generated as in Figure 2A and B comparing patterns of UEA-1, MHCII, K8 and K5 expression in 1-week-old vs. 6-week-old wild type mice as shown. (B) Quantification of the total numbers of TEC subpopulations in 1-week old and 6-week-old wild type mice within the total gated populations was performed by flow cytometry. Bar graphs represent mean+SEM with n = 9 from three independent experiments. (C) Frozen thymic sections from 1- and 6-week-old wild type were stained with anti-Aire FITC and rhodamine-conjugated UEA-1 and analyzed by fluorescence microscopy. Scale bar = 100 µm.