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. 2014 Jan 21;9(1):e86129. doi: 10.1371/journal.pone.0086129

Figure 8. RANKL differentially regulates TEC expansion in the post-natal vs. mature thymus.

Figure 8

(A) UEA/MHCII→K8/K5 (blue dots) and K8/K5→UEA/MHCII (red dots) overlays generated as in Figure 2A and B comparing patterns of UEA-1, MHCII, K8 and K5 expression in 1- vs. 6-week-old RANKL-Tg mice. (B) Quantification of the total numbers of TEC subpopulations in 1- and 6-week-old RANKL-Tg type mice was performed by flow cytometry. Bar graphs represent mean+SEM with n = 9 from three independent experiments. (C) Frozen thymic sections from 1- and 6-week-old RANKL-Tg mice were stained with anti-Aire FITC and rhodamine-conjugated UEA-1 and analyzed by fluorescence microscopy. Scale bar = 100 µm.