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. 2014 Jan 21;9(1):e86330. doi: 10.1371/journal.pone.0086330

Figure 2. SHP-1 regulates Nanog expression in F9 cells.

Figure 2

F9 cells transfected with pCMV-MYC-SHP-1-V1/V2, DLR assays, qPCR and western blotting were performed to detect the Nanog promoter activity (A) mRNA levels (B) and protein level (C); F9 cells transfected with si-SHP-1, the SHP-1 expression after silencing was detected (D) and DLR assays, qPCR and western blotting were performed to detect the Nanog promoter activity (E)mRNA levels (F) and protein levels (G). All data are presented as the mean +/− SD and are derived from three independent experiments. *P<0.05; **P<0.01; WB: western blot.