Fig. 3.

Reduction in the mitochondria complex I, II, and IV activities in idiopathic and Parkin-defect immortalized hAD-MSCs. (A) The activities of ETC enzymes (complex I, II, and IV) and citrate synthase were measured in mitochondria isolated from the immortalized non-PD, idiopathic and Parkin-defect cells. Compared to wild type cells, idiopathic cells showed significant decreases in mitochondria complex I, II, and IV activities by 52.7%, 44.2%, and 51.8%, respectively. Moreover, Parkin-defect cells showed decreases by 34.9%, 39.9%, and 39.0% in mitochondria complex I, II, and IV activities, respectively. All values are the means±SEM from three independent experiments. ^****p<0.0001, ^***p<0.001, CPXI (NADH dehydrogenase), CPXII (Succinate dehydrogenase), CPXIV (Cytochrome c oxidase). (B) Western blot analysis of prohibitin expression in mitochondria isolated from the immortalized wild type, idiopathic and Parkin-defect cells.