Figure 6. Images of the transgenic tobacco lines and the anthocyanin content, and the expression of anthocyanin biosynthetic pathway structural and regulatory genes in transgenic tobacco lines.

A, Images of tobacco lines containing the empty vector (wild type) or the LcMYB1 allele with the CaMV 35S promoter (Line 1–2) (Scale bars for images = 1cm. B, Anthocyanin content in different organs of transgenic tobacco lines. C, Regulatory gene expression in the anthocyanin biosynthetic pathway in transgenic tobacco. D, Structural gene expression in the anthocyanin biosynthetic pathway in transgenic tobacco lines. The Lcactin gene was used to normalize gene expression of the genes under identical conditions. The vertical bars represent the standard error of triplicate experiments.