Figure 2. Morpholino knockdown of the Nogo co-receptors p75 and TROY resulted in defects in neuromast formation in the zebrafish lateral line.

Zebrafish embryos at the 2∼4-cell stage were injected with morpholinos (MOs) against zebrafish p75 (B), TROY (C), or LINGO-1 (D). Neuromasts in these morphants were stained with 4-Di-2-ASP at 72 h post-fertilization (hpf). An embryo injected with control MO is shown in panel (A). Images were taken from the lateral view, and the yellow dots indicate neuromasts of the lateral line. Scale bar, 100 µm. A summary of PLL neuromast numbers in MO-injected embryos is shown in panel (E). Sample size (N) and MO dosage used are indicated.