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. 2014 Jan 21;9(1):e86367. doi: 10.1371/journal.pone.0086367

Figure 2. CGRP affects cytokine production in DC-Th cell interaction.

Figure 2

(A) The concentrations of each cytokine were determined by ELISA after 24 h stimulation with OVA and anti-CD40 mAb. Values are presented as means ± SD (n = 3). **P<0.01 VS. OVA+anti-CD40 mAb. (B) The mRNA expression was determined by real-time PCR after 6 h stimulation with OVA and anti-CD40 mAb. Values are presented as mean ± SD (n = 3). *P<0.05, **P<0.01 vs. OVA+anti-CD40 mAb. (C) BMDCs and DO 11.10 Th0 cells were co-cultured in the presence of 200 µg/ml OVA for 72 h. IFN-γ production was detected by FACS after intracellular staining. Data are represented of three independent experiments. α: αCGRP (1 nM), β: βCGRP (1 nM), dbc: db-cAMP (100 µM), 6b: 6-bnz-cAMP (100 µM), and 8C: 8-CPT-cAMP (100 µM) were added from start point of stimulation.