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. 2014 Jan 21;9(1):e86424. doi: 10.1371/journal.pone.0086424

Figure 4. Effect of 18 Gy Irradiation on Pax7 Cell Density in Limb and Extraocular Muscles from Wild Type and Het Mice.

Figure 4

A. Cross-section of non-irradiated het leg muscle immunostained for Pax7-positive cells, indicated by black and white arrows. B. Cross-section of het leg muscle 7 days after 18 Gy irradiation and immunostained for the presence of Pax7-positive cells. No positive cells can be seen. C. Cross-section of non-irradiated het EOM immunostained for the presence of Pax7-positive cells, an example indicated by white arrow. D. Cross-section of het EOM 7 days 7 days after 18 Gy irradiation and immunostained for the presence of Pax7-positive cells. All the black nuclei are Pax7-positive cells. Bar is 20 µm. E. Effect of 18 Gy irradiation on Pax7-positive cell density in leg muscle *indicates significant difference from non-irradiated WT control and non-irradiated het control. F. Effect of 18 Gy irradiation on Pax7-positive cell density in EOM from WT and het mice. *indicates significant difference from non-irradiated WT control and non-irradiated het control. # indicates significant difference from non-irradiated WT orbital control.