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. 2014 Jan 21;9(1):e86564. doi: 10.1371/journal.pone.0086564

Figure 7. AGE formation on HCAEC lysates following incubation with MGO.

Figure 7

Pooled HCAEC lysates from 4 donors (1 mg protein/mL) were incubated with 0–5 mM MGO for 1 h in PBS, with the formation of AGE determined by ELISA after cell lysis: (A) hydroimidazolones; (B) CEL, and; (C) CML. Data are means+SEM of AGE formation for 3 independent incubations, each measured in duplicate ELISA wells. *Significant change in activity (P<0.05) compared to 0 mM control activity (one-way ANOVA with Tukey’s post-test).