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. 2014 Jan 21;9(1):e86564. doi: 10.1371/journal.pone.0086564

Figure 8. HPLC identification and quantification of AGE formation on HCAEC lysate proteins following incubation with MGO.

Figure 8

HCAEC lysates (1 mg protein/mL) were incubated for 3 h with 0–5 mM MGO, followed by HPLC separation and analysis. (A) MG-H1, (B) MG-H2a, (C) MG-H2b, (D) CEL. Data are means+SEM of 3 independent experiments performed in duplicate. Statistical analysis was by paired t-test for (B) and (D), and Wilcoxon’s sign-ranked tests for (A) and (C), as the control values were below the limit of detection (∼13 µmol/mol Ser for MG-H1, and ∼18 µmol/mol Ser for MG-H2b), and must therefore be considered non-Gaussian. *Significant change (P<0.05) compared to 0 mM control.