Figure 2. Kinetic constants of HRP catalysis in presence of denaturants.

Relative kinetic constants of ABTS oxidation by solution phase HRP (white bars) and HRP encapsulated in 0.5% agarose (gray bars) and 2% agarose (black bars) in the presence of 35% ethanol and isopropanol – (a) V_max and (b) V_max/K_M. The relative kinetic constants were calculated by normalizing the kinetic parameters of HRP in the presence of denaturants to those in buffer. Error bars indicate the standard deviation of triplicate measurements.