Figure 3. Structural stability of enzymes encapsulated in agarose gels.

ANS fluorescence intensities (in arbitrary units) for solution phase enzyme (white) and enzyme encapsulated in 0.5% agarose (gray) and 2% agarose (black) exposed to 35% ethanol – (a) HRP and (b) β-gal. Fluorescence intensities for the agarose-protein formulations are reported after subtraction of the spectra of the corresponding agarose gels containing no enzyme.