Figure 4. Structural stability of HRP encapsulated in agarose-alginate hybrid gels.
ANS fluorescence intensities (in arbitrary units) for HRP encapsulated in agarose-alginate hybrid gels exposed to 35% ethanol – (a) 0.5% agarose (gray) and (b) 2% agarose (black) containing 0% (circles), 0.25% (squares), or 0.5% (triangles) alginate. Fluorescence intensities for the agarose-alginate-protein formulations are reported after subtraction of the spectra of the corresponding agarose-alginate gels containing no enzyme.