Table 2. Kinetic constants of ABTS oxidation by solution phase and agarose encapsulated HRP in 35% ethanol.
Condition | Vmax (µM s−1) | KM (µM) |
Solution phase | 1. 3±0.3 | 8.1±0.4 |
0.5% agarose | 3.1±0.4 | 8.7±0.6 |
2% agarose | 5.5±0.4 | 11.1±1.2 |
The data are the averages of three replicates and the error represents the standard deviation.