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. 2014 Jan 7;13:2. doi: 10.1186/1475-2859-13-2

Table 3.

Specific activities of AlsS, IlvC, IlvD, Kivd in cell extracts of Pseudomonas sp. strain VLB120, Pseudomonas sp. strain T7 pPAPC-Km, pPDPK and Ps. sp. strain VLB120 pCom10-kivd

  Specific activity (μmol min -1 mg total protein -1 )
 
 AlsS
 IlvC
 IlvD
 Kivd
VLB120 wild type
 0.033 ± 0.009
 0.0257 ± 0.0017*
 0.0075 ± 0.0004
 ND
VLB120 T7 pPAPC-Km, pPDPK
 3.494 ± 0.299
 0.1476 ± 0.0425*
 0.1955 ± 0.0023
 2.96 ± 0.24
VLB120 pCOM10-kivd n.d. n.d. n.d. ND

ND: not detectable.

*: coupled assay together with AlsS.

Cells were cultured in M9* pH 7.4, 20 g L-1 glucose and. 5 g L-1 yeast extract shake flasks at 30° Cells were induced for 4 h either with 0.05% DCPK (pCOM10) or with 1 mM IPTG (pPDPK, pPAPC-Km) in the early exponential phase. Errors indicate standard deviations (n = 2 - 3).