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. Author manuscript; available in PMC: 2015 Jan 15.
Published in final edited form as: J Immunol. 2013 Dec 13;192(2):630–640. doi: 10.4049/jimmunol.1302550

Figure 6.

Figure 6

Changes in number (left panels) and frequency (right panels) of thymocyte subpopulations in the absence of costimulatory interactions. Thymocytes from 4 week old BALB WT, CD80/86 KO, CD40 KO and CD40/CD80/86 KO mice were stained with anti-CD3 and CD1-PBS-57 tetramers to identify iNKT cells (top panels), or anti-CD4, anti-CD8 and anti-FoxP3 to identify Treg cells (bottom panels) and analyzed by flow cytometry. Bar graphs show the mean ± SE for 4-9 mice per strain. (*, p≤ 0.05; **, p≤ 0.01; ***, p≤ 0.001 ****, p≤ 0.0001).