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. 2014 Jan 22;4:3819. doi: 10.1038/srep03819

Figure 2. miR-143 inhibits ADSC differentiation when overexpressed during the first 2 days after adipogenic induction.

Figure 2

(A) Abundant lipid droplets formed on day 7 after adipogenic induction and were detected by Oil Red O staining. Bar, 100 μm. (B) and (C) The relative C/EBP-α and PPAR-γ mRNA levels were determined during differentiation by RT–qPCR at the time points indicated. (D) The protein levels of C/EBP-α and PPAR-γ were determined on day 7 after induction by western blot analysis. All measurements shown are the means ± SD of three independent experiments. **P < 0.01 vs untreated cells or cells transfected with the empty vector.