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. 2014 Jan 22;4:3819. doi: 10.1038/srep03819

Figure 3. Overexpression of miR-143 during the first 2 days after adipogenic induction impaires the clonal expansion of ADSCs.

Figure 3

(A) and (B) Cell-cycle analysis of ADSCs using flow cytometry 24 h after induction. (C) ADSC proliferation was monitored with an MTT assay for 4 days after induction. (D) Fluorescence attributable to BrdU incorporation by ADSCs. Bar, 50 μm. (E) Quantification of BrdU incorporation in cells 24 h after induction, determined with a cell-proliferation ELISA kit. All measurements shown are the means ± SD of three independent experiments. *P < 0.05, **P < 0.01 vs. untreated cells or cells transfected with the empty vector.