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. 2014 Jan 22;4:3819. doi: 10.1038/srep03819

Figure 4. miR-143 promotes ADSC differentiation when overexpressed 2 days after induction.

Figure 4

(A) After reaching confluence, ADSCs infected with miR-143-expressing lentiviral vector and untreated cells were incubated with (+) or without (−) MDI for 7 days and then stained with Oil Red O. Bar, 100 μm. (B) and (C) The relative C/EBP-α and PPAR-γ mRNA levels were determined with RT–qPCR during differentiation at the time points indicated. (D) Active PPAR-γ (PPAR-γ) and inactive phosphorylated PPAR-γ (p-PPAR-γ) concentrations were determined in each group with western blot analyses on days 3 and 5 after differentiation. (E) Proliferation of the ADSCs in (A) was tested for 4 days after induction with an MTT assay. (F) BrdU incorporation by the cells in (A) was quantified 24 h after induction. (G) and (H) Cell cycle of ADSCs was analyzed by flow cytometry 24 h after induction. Data shown are the means ± SD of three independent experiments. **P < 0.01 vs. untreated cells.