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. 2014 Jan 16;15:29. doi: 10.1186/1471-2164-15-29

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Copyright © 2014 Singh et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Validation of differential expression (fold change) of two randomly chosen candidate genes (pre-α/β-gliadin and γ-Gliadin) at three seed development stages using qRT-PCR. The validation was done between a good (C306) and a poor (Sonalika) chapatti quality varieties in 7, 14, and 28 days after anthesis (DAA) of seed developmental stage through quantitative real time PCR (qRT-PCR). Y-axis represents fold change in differential expression of genes in the wheat variety, C306 in comparison to the wheat variety, Sonalika. X-axis represents three seed development stages i.e. 7, 14, and 28 DAA. The expression data were normalized to that of a control gene, ADP ribosylation factor, ARF. qRT-PCR data analysis was done following Livak and Schmitteng (2001) [46].