Table 5.

Nucleotide sequences of forward and reverse primers of two randomly chosen candidate genes for processing quality (pre-α/β-gliadin and γ-Gliadin) and one control (ADP ribosylation factor, ARF) used for their validations of differential expression between good and poor quality varieties using quantitative real time PCR (qRT-PCR)

Gene	Forward primer (5′-3′)	Tm (°C)	Reverse primer (5′-3′)	Tm (°C)	Product size (bp)
ADP ribosylation factor (ARF)	TGATAGGGAACGTGTTGTTGAGGC	57.4	AGCCAGTCAAGACCCTCGTACAAC	59.1	200
Pre-α/β-gliadin	GACCTTTCTCATCCTTGTCCTCCT	57.4	CTGTGAATATGGTAGTTGCGGCTG	57.4	265
γ-Gliadin	TCTCTACAACAACAGATGAACCCCTG	58.0	GCCTTGTTGTTGTTCTTGCTGCATG	57.7	225