Table 6.
Fold change values of the validated (by qRT-PCR) pre-α/β-gliadin and γ-gliadin genes between good and poor quality varieties at three seed development stages using qRT-PCR and microarrays
| Gene/seed development stage* |
Fold change values in gene expression between C306 and Sonalika |
|
|---|---|---|
| qRT-PCRs | Affymetrix wheat microarrays | |
|
pre α/β-gliadin | ||
| 7 DAA |
74.7 |
100.7 |
| 14 DAA |
1.0 |
-1.0 |
| 28 DAA |
1.4 |
-1.2 |
|
γ-gliadin | ||
| 7 DAA |
47.6 |
38.8 |
| 14 DAA |
6.4 |
-1.4 |
| 28 DAA | 1.7 | -2.0 |
*Seed development stages at 7 DAA (days after anthesis), 14 DAA, and 28 DAA.