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. 2014 Jan 17;10:3. doi: 10.1186/1744-8069-10-3

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Copyright © 2014 Yasaka et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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VGLUT2 in the axon of one of the vertical cells from the CTb injected animals. a, A projected confocal scan (51 z sections at 1 μm spacing) showing the morphology of one of the delayed-firing vertical cells (cell 10 in Table 1). The axon emerges from the right side of the soma (arrow) and gives off a single collateral (arrowhead), before leaving the slice. This collateral gives rise to branches with numerous boutons. The dashed line indicates the dorsal edge of the VGLUT1 plexus. b, A projection of 4 confocal optical sections (0.5 μm z-spacing) scanned to reveal Neurobiotin (NB) shows five boutons (arrowheads) belonging to the axon of this cell (some of those shown in the boxed area in a). c, The same field scanned to reveal VGLUT2. d, A merged image reveals that each of these boutons is VGLUT2-immunoreactive. Scale bars: 100 μm (a), 10 μm (b-d).