Fig. 2.

Function of abcA expressed in S. cerevisiae. Plasmids directing the expression of either abcA-GFP or Fur4-GFP from the CUP1 promoter were introduced into the indicated S. cerevisiae strains. (A) Transformants were grown to mid-log phase and then placed in duplicate on YPD medium containing 0.5 μg/ml fluconazole. Two identical plates were prepared and then placed at either 30 °C or 37 °C as noted. These plates were incubated for 48 h and then photographed. (B) Reversal of resistance phenotypes caused by loss of Pdr5. A pdr5Δ yor1 strain was used to enhance the relevant phenotypes as described (Kolaczkowska et al., 2008; Johnson et al., 2010). Transformants from above were placed on YPD medium containing the indicated concentrations of 4-nitroquinoline-N-oxide (4-NQO) or phytosphingosine (PHS). Resistance to these compounds is elevated in the absence of Pdr5 (Kolaczkowska et al., 2008). Transformants were incubated at 37 °C and the plates photographed.