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. 2013 Jun 17;32(49):5512–5521. doi: 10.1038/onc.2013.217

Figure 4.

Figure 4

GW9662 toxicity in ERBB2-positive CSCs is because of production of ROS. (a) Flow cytometry analysis of ROS production. BT474 cells treated with 10 μM GW9662 or 1 mM L-S,R- BSO for 9 days, and intracellular ROS concentration were assessed by DCF-DA staining. (b) DCF-DA fluorescence in BT474 cells presented as mean fluorescence intensity. Error bars indicate s.d. (n=3), *P<0.001, **P<0.05. (c) BT474 cells were treated with vehicle, 10 μM GW9662 or 1 mM BSO together with 1 mM of NAC for 9 days. Live cells were counted and presented as percentage of the vehicle control (*P<0.05, **P<0.001). (d) % of ALDH-positive cells of BT474 cells treated with vehicle, 10 μM GW9662 or 1 mM BSO together with 1 μM NAC for 9 days. Error bars indicate the s.d. from three individual experiments. *P<0.05, **P<0.001.